multimode fluorescence microplate reader  (Revvity)

Journal: Frontiers in Pharmacology

Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

doi: 10.3389/fphar.2026.1655883

Figure Lengend Snippet: NXT-Exo protected HMEC-1 from LPS-induced inflammation, and oxidative damage. (A) LDH releases from HMEC-1 was detected by microplate method. (B–F) The secretion levels of IL-1β, IL-6, IL-8, VCAM-1 and ICAM-1 were measured via ELISA. (G,H) The expression levels of ROS and MDA were detected by fluorescence method. Values are expressed as mean ± SD, n = 3. * p < 0.05 and ** p < 0.01.

Article Snippet: Cells were detected under Spark fluorescence microplate reader (Tecan Spark, Switzerland).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Fluorescence

Journal: Frontiers in Pharmacology

Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

doi: 10.3389/fphar.2026.1655883

Figure Lengend Snippet: miR-382-5p downregulated the expression of STC1 and the effect of STC1 on the endothelial protection of NXT-Exo. (A) The mRNA expression level of STC1 in HMEC-1 transfected with miR-382-5p mimic or miR-382-5p mimic-NC was detected by RT-qPCR. (B) The mRNA expression level of STC1 in HMEC-1 treated with NXT-Exo or NXT-Exo combined with miR-382-5p was detected by RT-qPCR. (C) The transfection of si-STC1 was verified by RT-qPCR. (D,E) The secretion levels of IL-6 and IL-8 were measured by ELISA. (F) The release of LDH was detected by microplate method. (G,H) The production level of ROS and expression level of MDA were tested by fluorescence. (I) The effects of miR-382-5p and STC1 treatment on the relative protein expression levels of Bax, Bcl-2, Cleaved caspase 3 and STC1 in HMEC-1 were detected by Western blot. Values are expressed as mean ± SD, n = 3. *p < 0.05, **p < 0.01.

Article Snippet: Cells were detected under Spark fluorescence microplate reader (Tecan Spark, Switzerland).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Fluorescence, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Naoxintong capsule decreases circulating exosomes of miR-382-5p to protect LPS-induced vascular endothelial cell injury by targeting STC1 in vitro

doi: 10.3389/fphar.2026.1655883

Figure Lengend Snippet: NXT-Exo protected HUVEC from LPS-induced inflammation, and oxidative damage. (A) LDH releases from HUVEC was detected by microplate method. (B–F) The secretion levels of IL-1β, IL-6, IL-8, VCAM-1 and ICAM-1 were measured via ELISA. (G,H) The expression levels of ROS and MDA were detected by fluorescence method. Values are expressed as mean ± SD, n = 3. * p < 0.05, ** p < 0.01.

Article Snippet: Cells were detected under Spark fluorescence microplate reader (Tecan Spark, Switzerland).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Fluorescence